Written in English
This thesis presents the development of a system for single cell manipulation using radiation pressure generated forces. Two methods of cell manipulation techniques were investigated: laser tweezing and laser guiding. In laser tweezing, single mode tensed fiber, microfabricated using a molecular fluorine (F2) excimer laser system, were used to trap live biological cell. The lens fibers were designed according to an ASAP2005 optical simulation using beam propagation method (BPM) and a MatLab surface profile modelling. In the second part, an integrated microfluidic device was designed and fabricated for the cell guiding experiment. The device consists of microfluidic channels interconnected with optical waveguides. The radiation pressure carried by the light propagating in the waveguide deflected cells into a transverse microfluidic channel perpendicular to the original direction of flow.
|The Physical Object|
|Number of Pages||104|
The volumetric power density of this cell with the cell volume cm 3 was estimated to be mW cm −3. The stability of the power output and that of the device itself are however not known. Chen and colleagues from the University of Houston later developed a thin-film SOFC on a 6-μm-thick polycrystalline nickel foil. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~ events per second without the need for fluorescent labeling. Single cell manipulation tools integrated with microwells. (a) Optical tweezers: a cell of interest is levitated and collected from the microwell by applying a highly focused laser beam. (b) Immunomagnetic sorting: cells of interest are sorted from the heterogeneous population by forming a cell-magnetic bead complex. The content of this chapter is broadly divided into two parts—single-cell manipulation (SCM) and single-cell analysis (SCA). The first part of the chapter presents state-of-the-art techniques developed to handle single cells, including counting, sorting, positioning, and culturing, which are essential steps in many biological and medical assays.
Microfabricated and microfluidic devices enable standardized handling, precise spatiotemporal manipulation of cells and liquids, and recapitulation of cellular environments, tissues, and organ. 9 Microﬂ uidic devices for single-cell trapping and automated micro-robotic injection X. Y. Liu, McGill University, Canada and Y. Sun, University of Toronto, Canada Introduction Device design and microfabrication Experimental esults r and discussion Conclusion (1,2) On the other hand, single-cell manipulation is also regarded as an important elemental technique in cell analysis. Optical tweezers using laser light are widely employed for the manipulation of microbial cells.(3–6) The principle of a single-beam gradient force trap is, in essence, optical levitation by radiation pressure.(7) In a typical. Trapping/immobilization of single biological cells into a regular pattern is an important cell manipulation procedure and has applications in many single-cell-based studies, such as molecule/drug screening (Castel et al., ), fate/function studies (Chen and Davis, ), cell pairing/fusion (Skelley et al., ), and DNA damage analysis.
The book gives a history of miniaturization and micro- and nanofabrication, and surveys industrial fields of application, illustrating fabrication processes of relevant micro and nano devices. In this second edition, a new focus area is nanoengineering as an important driver for the rise of novel applications by integrating bio-nanofabrication. The technique has potential applications in cell manipulation, [15, 16] separation, [17,18,19,15] sorting, [20,21,22] to study electrorotation, [23,24] electrofusion, [25,26,27] cell-cell. The atomic force microscope  was invented in the s to reach beyond the limits of the scanning tunneling microscope  by obtaining atomic-resolved images not only of conductive monocrystalline surfaces but also of insulating surfaces. Atomic force microscopy (AFM) in its most standard configuration features a pyramidal tip at the extremity of a flexible cantilever that acts as a spring. Several single-cell analysis techniques have been developed, which may be classified in terms of information content (# of elements capable of being studied simultaneously) and throughput (# of cells studied in a given time) – this is illustrated in Fig. simplest and most widely used forms of single-cell analysis are fluorescence microscopy and flow cytometry.